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plasma et 1 concentrations  (R&D Systems)


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    R&D Systems plasma et 1 concentrations
    Plasma Et 1 Concentrations, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 243 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 243 article reviews
    plasma et 1 concentrations - by Bioz Stars, 2026-03
    95/100 stars

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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by <t>ELISA</t> (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.
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    Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of <t>ET-1</t> (D), and aldosterone (E) during ad libitum feeding and RF in WT mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA indicated significant Feeding × Time interactions ( P < 0.05; bars indicate significant post hoc comparisons).
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    Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by ELISA (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.

    Journal: Communications biology

    Article Title: Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis.

    doi: 10.1038/s42003-023-05057-w

    Figure Lengend Snippet: Fig. 6 Spd suppresses fibrotic signaling, but does not inhibit the endothelin pathway like bardoxolone methyl. a Secretion of TGFβ1 from HK-2 cells treated with Spd measured by ELISA (n = 6 in each group). Data are indicated as means ± SD. b Collagen 1 and c HO-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with TGFβ1 in the presence of Spd (n = 3 in each group). Data are indicated as means ± SD. d MitoTracker Red CMXRos staining in HK-2 cells when exposed to H2O2 and Spd. Scale bar, 100 µm. e Quantification of Mitotracker Red CMXRos intensity measured by a multimode plate reader (n = 8 in each group). Data are indicated as means ± SD. f HO-1 and g ET-1 mRNA expression determined by real-time PCR in HK-2 cells incubated with Spd and CDDO-me (n = 3 in each group). Data are indicated as means ± SD. h Secretion of ET-1 from HK-2 cells treated with Spd and CDDO-me measured by ELISA (n = 3 in each group). Data are indicated as means ± SD. **P < 0.01. NS not significant, Spd spermidine, TGFβ1 transforming growth factor β1, CDDO-me bardoxolone methyl.

    Article Snippet: To measure TGFβ1 and ET-1 concentrations in the supernatant, we used the human TGFβ1 Quantikine ELISA kit (DB100B; R&D Systems, Minneapolis, MN, USA) and the human ET-1 Quantikine ELISA kit (DET100; R&D Systems).

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Real-time Polymerase Chain Reaction, Incubation, Staining

    Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of ET-1 (D), and aldosterone (E) during ad libitum feeding and RF in WT mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA indicated significant Feeding × Time interactions ( P < 0.05; bars indicate significant post hoc comparisons).

    Journal: Function (Oxford, England)

    Article Title: Timing of Food Intake Drives the Circadian Rhythm of Blood Pressure

    doi: 10.1093/function/zqaa034

    Figure Lengend Snippet: Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of ET-1 (D), and aldosterone (E) during ad libitum feeding and RF in WT mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA indicated significant Feeding × Time interactions ( P < 0.05; bars indicate significant post hoc comparisons).

    Article Snippet: Urinary endothelin-1 (ET-1) concentrations were determined by ELISA (QuantiGlo ET-1 Kit, R&D Systems, Minneapolis, MN).

    Techniques:

    Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of ET-1 (D), and aldosterone (E) during ad libitum feeding and RF in Bmal1KO mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA was used.

    Journal: Function (Oxford, England)

    Article Title: Timing of Food Intake Drives the Circadian Rhythm of Blood Pressure

    doi: 10.1093/function/zqaa034

    Figure Lengend Snippet: Urine volume (A), urinary sodium excretion (UNaV; B), urinary potassium excretion (UKV; C), urine excretion of ET-1 (D), and aldosterone (E) during ad libitum feeding and RF in Bmal1KO mice. Data are presented as 12-h average of the last 2 days. Repeated measures two-way ANOVA was used.

    Article Snippet: Urinary endothelin-1 (ET-1) concentrations were determined by ELISA (QuantiGlo ET-1 Kit, R&D Systems, Minneapolis, MN).

    Techniques: